Tartrate-Resistant Acid Phosphatase (TRAP) Staining Kit

Acid phosphatase (ACP) is widely distributed in virtually all tissues, predominantly within lysosomes, and is therefore commonly used as a lysosomal marker enzyme. Extralysosomal ACP is also present in the endoplasmic reticulum and cytoplasm. ACP isoforms differ among species; the optimum pH is 4.5–5.5. Tartrate-resistant acid phosphatase (TRAP) is found in follicles of normal human alveolar macrophages and in leukemic spleen cells, but is not released into blood. Consequently, serum TRAP activity largely reflects osteoclast function, enabling assessment of osteoclastic status via blood measurement.EnkiLife TRAP Staining Solution uses naphthol AS-BI as substrate; under acidic pH, ACP hydrolyses the substrate to release phosphate and naphthol, which couples with a diazonium salt to yield a colored product localized in the cytoplasm. Cells whose ACP is resistant to tartrate exhibit a positive reaction. Suitable for fresh blood smears, cytospins, and frozen sections. Storage: store at -20°C for 6 months, keep away from light For more information, you can visit the product URL: https://www.enkilife.com/p/Tartrate-Resistant-Acid-Phosphatase-TRAP-Staining-Kit-RA20118