Prussian Blue Staining Kit (Nuclear Fast Red Counterstain Method)

Hemosiderin is a hemoglobin-derived pigment that appears as golden or brownish-yellow granules. Due to its iron content and golden color, it is referred to as hemosiderin. When red blood cells are phagocytosed by macrophages, hemoglobin is broken down into iron-free orange chromogen and iron-containing hemosiderin under the action of lysosomal enzymes. The Prussian blue reaction, also known as hemosiderin staining, produces a blue color after treatment with potassium ferrocyanide and dilute acid. It is commonly observed in phagocytes or interstitial tissues. The staining principle is that potassium ferrocyanide solution, in the presence of dilute hydrochloric acid, dissociates trivalent iron from proteins. The trivalent iron then reacts with potassium ferrocyanide to form an insoluble blue compound—ferric ferrocyanide.EnkiLife Prussian Blue Staining Solution (Nuclear Fast Red Counterstain Method) is used to visualize various hemorrhagic lesions in local tissues, commonly seen in phagocytes. It effectively distinguishes hemosiderin from other pigments. This staining solution offers good stability, long-term storage capability, minimal precipitation, and a wide range of applications. After the reaction, a red counterstain such as nuclear fast red, eosin, or neutral red may be used. Nuclear fast red is used as the counterstain in this kit, representing the most classic and commonly used method. Storage: store at RT for 12 months For more information, you can visit the product URL: https://www.enkilife.com/p/Prussian-Blue-Staining-Kit-Nuclear-Fast-Red-Counterstain-Method-RA20122