NADP+/NADPH Assay Kit (WST-8 Method)

This kit is a colorimetric kit based on WST-8, which detects the amount, ratio and total amount of NAD + (oxidized coenzyme I) and NADH (reduced coenzyme I) in cells, tissues or other samples by colorimetry. NAD ( Nicotinamide adenine dinucleotide ) is a coenzyme present in all cells, including two forms: NAD + ( oxidized form ) and NADH ( reduced form ) . NAD + is not only a coenzyme that transfers electrons during redox reactions, but also can be used as a substrate for many enzymes to participate in intracellular reactions. For example, deacetylases such as Sirt1 of the Sirtuins family need NAD + as a substrate for deacetylation reactions to regulate the acetylation level of proteins and thus participate in the life activities of cells. NAD + plays an important role in cells and the body. Its synthesis and degradation and its products are involved in cell apoptosis, metabolic regulation and gene expression regulation, and the reduction of NAD + is one of the main factors for cell death. The importance of NAD + in regulating the cellular redox state and its function in regulating signaling pathways and transcription make NAD + and the enzymes that synthesize and consume it potential drug targets for a variety of diseases Storage: The unopened kit can be stored at -20°C for 12 months. For more information, you can visit the product URL: https://enkilife.com/product/view/14969