Modified Van Gieson (VG) Staining Kit

Collagen fibers are the most widely distributed and abundant fibers in connective tissue, found extensively in various organs, especially in the skin, sclera, and tendons. The principle of Van Gieson collagen fiber staining is related to the size of anionic dye molecules and tissue permeability. Molecular size is reflected by molecular weight: smaller molecules penetrate dense, low-permeability tissues more easily, while larger molecules can only enter loose, high-permeability tissues. Phosphomolybdic acid (PA) has the smallest molecular weight, followed by acid fuchsin and ponceau, while light green has the largest. After VG staining, muscle fibers appear yellow, and collagen fibers appear red.EnkiLife Modified Van Gieson (VG) Staining Solution uses Celestine Blue and Mayer’s Hematoxylin for nuclear staining, resulting in better staining quality and longer storage stability. Ponceau S is used for collagen staining, which is fade-resistant. It is commonly used to distinguish collagen fibers from muscle fibers, to differentiate collagen-derived tumors from muscle-derived tumors, and to observe tissue or organ damage, repair, and fibrosis. Storage: store at 2-8°C for 12 months, keep away from light For more information, you can visit the product URL: https://www.enkilife.com/p/Modified-Van-Gieson-VG-Staining-Kit-RA20135