Modified Oil Red O Staining Kit

Lipids are a general term for neutral fats, lipoids, and their derivatives. Their common physical property is insolubility in water and solubility in organic solvents (e.g., ethanol, ether). Human fat mainly includes: Storage fat, such as neutral fat, primarily distributed in subcutaneous tissue, kidneys, pancreas, etc.; Structural fat, such as lipoids (phospholipids, glycolipids, cholesterol), mainly found intracellularly. Neutral fat is composed of three fatty acid molecules and one glycerol molecule. It is neutral and serves as one form of energy storage, releasing energy upon oxidation. Traditional neutral fat staining methods include Sudan II, Sudan III, Sudan IV, Sudan Black B, and Oil Red O. Recent studies have shown that the azo dye Oil Red O is more suitable for fat staining. Oil Red O is a strong lipid solvent and lipophilic dye, easily forming small lipid droplets with triglycerides, though its affinity for phospholipids is slightly weaker. The staining mechanism is generally considered a physical dissolution or adsorption process. The dye dissolves in lipids within frozen sections more readily than in the original solvent, thus transferring from the organic solvent into the lipid, resulting in fat staining.EnkiLife Modified Oil Red O Staining Solution is primarily used to demonstrate fatty degeneration and abnormal lipid deposition in tissues and organs, commonly seen in fatty degeneration of parenchymal organs such as liver, kidney, and heart, where numerous neutral fat droplets appear intracellularly. It is also used for the identification and diagnosis of tumors in adipose tissue and their nature. Positive fat staining appears orange-yellow to red, with exact color depending on lipid concentration. Storage: store at 2-8°C for 12 months For more information, you can visit the product URL: https://www.enkilife.com/p/Modified-Oil-Red-O-Staining-Kit-RA20128