Fluo-20 AM Calcium Probe(Green)

Calcium measurement plays a vital role in various biological research fields. By utilizing fluorescence probes that emit spectral responses upon binding to Ca²⁺, researchers can study intracellular free Ca² concentration changes through instruments like fluorescence microscopes, flow cytometers, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are the most commonly used visible light-excited calcium indicators in live cell imaging research. However, these two probes have certain limitations: after esterase hydrolysis, they only exhibit moderate fluorescence intensity in living cells. Moreover, achieving optimal cellular calcium response requires stringent cell loading conditions. The emergence of Fluo-20 AM effectively addresses these issues. While maintaining similar spectral wavelengths (Ex/Em = 495/516 nm) and ease of use as Fluo-3 AM, Fluo-4 AM, and Fluo-8 AM, it significantly improves cell loading conditions and calcium response efficiency. For instance, Fluo-20 AM can complete cell loading at room temperature, whereas Fluo-3 AM and Fluo-4 AM require a 37℃°C environment for loading. Additionally, Fluo-20 AM demonstrates four times the brightness of Fluo-4 AM, eight times that of Fluo-3 AM, and twice that of Fluo-8 AM. Fluo-20 AM enables easy detection of weak calcium ion concentration changes while minimizing background interference, making it particularly suitable for scenarios with weak signals such as primary cells or calcium-sensitive cells. As an acetyl methyl ester derivative with cell membrane permeability, it can easily enter cells through simple culture. Once inside the cell, it is cleaved by endolysin into non-permeable Fluo-20, which remains within the cell to perform physiological functions. In summary, Fluo-20 AM serves as an ideal replacement for Fluo-3 AM, Fluo-4 AM, and Fluo-8 AM. Storage: -20°C（keep away from light） For more information, you can visit the product URL: https://www.enkilife.com/p/RA20088